Plasmid

Part:BBa_K4427009:Design

Designed by: Chanjuan Jin   Group: iGEM22_JLU_China   (2022-09-30)


pSB1C3-stuffer-pro


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2795
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2796
    Illegal PstI site found at 2810
    Illegal NotI site found at 7
    Illegal NotI site found at 2803
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 1939
    Illegal BamHI site found at 1165
    Illegal BamHI site found at 2717
    Illegal XhoI site found at 1743
    Illegal XhoI site found at 2726
    Illegal XhoI site found at 3827
    Illegal XhoI site found at 4719
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2796
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2796
    Illegal PstI site found at 2810
    Illegal NgoMIV site found at 1313
    Illegal AgeI site found at 1000
    Illegal AgeI site found at 2593
    Illegal AgeI site found at 2705
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

JLU_China


Source

yes

References